Pipette tips come in many shapes and sizes. Choosing the proper tip is crucial to achieving the desired precision and accuracy. If you choose the wrong tip, it could result in a number of problems, including waste of samples, reagents, and even repetitive stress injury. Luckily, there are many types of micropipette tips available for your use. Learn about the three most common types and which one you should use.
Most pipette tips are universal. This means that they fit all micropipettes on the market, including those made by other manufacturers. However, this can pose some challenges, since pipette barrels vary in diameter. To overcome these problems, companies focused on universal pipette tips developed new technologies, such as FlexFit, which helps the tips fit more pipette types and provide more accuracy.
The first characteristic to look for in a pipette tip is the polypropylene blend. A high-quality tip will be made of virgin polypropylene, which is free of plastic and metal additives. This will protect your samples and prevent cross contamination. Also, if you need a colored tip, make sure it does not contain metal additives. Dyes often contain metal compounds. If you're unsure, read the package label.
Low-retention micropipette tips are manufactured using a variety of methods. One technique is mechanical polishing, which renders the tips ultra smooth. This prevents liquid samples from sticking to the tip surface. The tips are also gamma-radiated to guarantee sterility. Investing in high-quality tips is worth the money. It can make or break your experiment. If you're serious about accuracy, precision, and ease of use, you'll pay for a high-quality tip.
Filtered micropipette tips are another popular choice. Filtered tips prevent aerosols from forming, which are tiny liquid/solid particles that remain airborne for long periods of time. Aerosols are the number one cause of contamination in laboratories. Filtered tips shield samples from pipette aerosols. This is especially important for ultrasensitive molecular biology applications. However, some filter tips may interfere with the flow of liquid while others can be dyed to prevent contamination. These tips are also not suitable for small-volume tests.
When choosing a pipette, consider the amount that you need to transfer. Fixed micropipettes have preset volume ranges, while variable micropipettes allow you to adjust the amount of liquid. The adjustable volume is a great choice for researchers and scientists who need to accurately measure and transfer volumes. The volume settings can be changed easily using the volume adjuster knob on the micropipette. The tips should not exceed the recommended volume ranges.
Air displacement pipettes are another type of micropipette. These pipettes use pistons that move upward when pushed by a plunger. As the piston is forced upward, the liquid surrounding the tip is displaced into the vacuum. This allows the sample to be transported or released in a controlled manner. However, these devices are prone to different types of user technique and environment. There is a wide range of tips available for micropipette tips.
The first consideration to make when purchasing a pipette is the type of tip that you require. The shapes of these tips vary from one another depending on their build quality and the manufacturer. While you can reduce this variability by buying good quality tips, you should also consider how much you will be using the pipette. If you don't need to repeat measurements, a flexible tip may be the best option. It also offers greater precision.
Pipette tips have evolved over the years. Manufacturers now ensure that the polypropylene they use is free of additives, 9-octadecenamide, and other chemicals that could compromise the quality of the sample. They have also improved their quality control by applying automated methods and innovative technologies. They use highly polished equipment and do not include demolding aids or parting agents. In general, pipette tips are safe to use in sensitive assays.
One of the most frequently asked questions that we get from lab technicians is, "Why does alcohol drop from a pipette tip?" The most common type of pipette has a bulb that can be blown out. Its tip is used to transfer the liquid from the flask.
Depending on the application, pipette tips can vary in length and style. Extended tips can be used in deep well blocks and microcentrifuge tubes. Short tips are more suitable for smaller wells. Short tips also improve ergonomics, as they allow the pipette to be used closer to the bench. This reduces strain on the arm. The GLP guide explains the different types of pipette tips and provides guidelines for choosing the right one.
There are two primary factors influencing tip fit. The diameter of the tip is not the only factor to consider. The connection between the pipette tip and the pipette must fit tightly to prevent air from escaping. If the fit is not snug enough, the pipette system may not aspirate enough liquid and it could leak. Consequently, this can lead to an inaccurate analysis result. In addition, it can cause an increase in the overall accuracy of the assay.
The quality of pipette tips can be affected by their composition. High-quality tips are made from virgin polypropylene without any plastic additives. Non-sterile tips are autoclaved, but this doesn't guarantee sterility. Depending on the application, macro tips are suitable for larger volumes of liquids and can handle five to ten ml of reagents. However, the price difference between these two types of tips can be considerable.
Different manufacturers make different types of pipette tips. These types differ based on the materials used and the design/shape. All of these types of tips will vary slightly in size and shape. The main factors affecting pipette tips include the manufacturer's reputation and the quality of the materials used. In addition to their size, pipette tips may also be compatible with other brands. Some manufacturers offer sterile tips for convenience.
Whether you choose a cheap or expensive tip, always consider the quality of the machine used for manufacturing them. Small batch-to-batch variations in the process can negatively affect the results of your study. Those irregularities may not be visible to the naked eye. You don't want to use tips that don't fit properly. You should also be aware that some tips may not fit your pipettes and vice versa. Hence, you should always perform gravimetric tests to determine the quality of the tip before using it.